Parallel Inducible Cell Based Kinase Screen

WO04061409

A parallel inducible cell-based kinase screen (PICKS) includes a plurality ofcells that express a kinase gene under the control of an inducer and a reporter gene, wherein the cells are derived from a single cell line, and wherein the reporter gene generates a signal in response to catalytic activity or inhibition of the expressed kinases. Particularly preferred systems include cells expressing kinases from a single kinase signaling pathway, kinases from a kinase family, and/or kinases from various different kinase signaling pathways. Consequently, contemplated systems provide a platform for screening for novel kinase inhibitors, inhibition specificity of particular kinase inhibitors, and for analysis of inter-pathway and/or intra-pathway inhibition of a kinase inhibitor.